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Application of Membrane Integration Technology in Enzymatic Peptide Production Process

There are many kinds of peptides in the human body, and the peptides dominate the life processes of human growth, development, reproduction and metabolism. The active polypeptide can promote cell division, ensure gene expression and replication, ensure that the quantity, quality and speed of protein synthesis in the cell are in a normal state, thereby controlling the growth and development of the human body, aging and disease.

The active polypeptide has the characteristics of being easily absorbed and utilized. The polypeptide can supplement the active substances and nutrients lacked in the body, thereby improving cell metabolism, restoring the disordered internal environment, and working in a healthy and coordinated manner between the various systems of the body. Not only can it quickly eliminate fatigue, but it can also prevent and cure many diseases such as diabetes and cardiovascular and cerebrovascular diseases. In addition, the polypeptide has a protective effect on cardiomyocytes and brain cells. Peptides have significant improvement in symptoms such as hyperlipidemia, hypertension, cerebral arteriosclerosis, coronary arteriosclerosis, atherosclerosis, coronary heart disease, cerebral thrombosis sequelae, palpitations, nausea, and palpitation.

Polypeptides, as the chief engineer of human cell conditioning, are far from enough if they rely solely on the body's own synthesis. Especially people with digestive and absorbing functions need to supplement peptides to maintain normal function of the human body.There are many methods for producing polypeptides in the industry, such as acid method, alkali method, electric method, artificial grafting method, gene expression method, biological fermentation method, enzymatic method and the like.

A method of catalyzing a protein with a biological enzyme is called an enzymatic method. The biological enzymatic degradation method utilizes the biological enzyme technology to obtain a polypeptide by enzymatic hydrolysis, degradation and hydrolysis of the protein, and the molecular weight distribution is between 200-1000 Da. The enzymatic process has the characteristics of simple process, small investment, stable peptide product quality, high activity, high content and good taste. In the enzymatic production process, the composition of the enzymatic hydrolysate is complex, and in addition to the polypeptide, it also contains impurities such as macromolecular proteins, enzymes, and ash. In order to obtain a product with higher purity, it is necessary to remove impurities as much as possible by a separation method to enrich the polypeptide. Finally, spray drying is carried out to make a finished product of high purity. The traditional separation method generally uses coarse filtration through centrifugation or plate frame to remove ineffective components such as macromolecular proteins and enzymes, and then removes water by evaporation or membrane concentration plus evaporation, and finally spray-drying. Traditional process applications are relatively early and relatively mature, but they also have shortcomings. The specific performance is that the filtration accuracy of the centrifuge or the plate frame is not high, and the macromolecular protein cannot be effectively removed. At the same time, because the impurity removal is not thorough enough, the membrane concentration of the latter channel is higher and the flux is lower. Moreover, the concentration of the membrane concentration is not high enough, and multi-effect evaporation is required to spray dry. The ceramic membrane separation technology is a separation method with high separation precision, stepwise gradient separation and green zero addition, which can meet the above separation requirements more efficiently and environmentally.

Membrane separation technology is a separation technique developed in recent decades. According to different membrane pore diameters, it can be divided into several different separation precisions such as ceramic microfiltration (MF), ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO). Due to its high efficiency of separation, it has been widely used in food, medicine, chemical, nano materials, new energy and environmental water treatment.

Membrane separation technology has the following characteristics:

1. It is a pure physical process, zero added, green and environmentally friendly;

2. The separation process only needs pressure driving; the process is simple and the degree of automation is high;

3. The phase separation does not occur during the separation process, and is particularly suitable for the separation of flavor and heat sensitive substances;

4. It can classify substances with different molecular weights; it uses a variety of pore size membranes to form gradient filtration, which can be separated and concentrated step by step.

The effective molecular weight of the polypeptide product is generally between 200 and 2000 Da. After proteolysis, the enzymatic hydrolysate was initially clarified by microfiltration. The microfiltration supernatant is then subjected to ultrafiltration to remove enzymes and macromolecular water-soluble proteins. The ultrafiltration supernatant is subjected to nanofiltration concentration while removing inorganic salts. The nanofiltration concentrate is then subjected to resin removal of heavy metals. The liquid after removal of the heavy metal is further concentrated by reverse osmosis. The reverse osmosis concentrate is directly dried by spray drying to obtain a finished product.

Advantages of membrane technology for peptide production:

1. The microfiltration plus ultrafiltration membrane technology has high filtration precision. Compared with the traditional centrifugal or plate-frame process, the impurity removal rate is higher, the filtrate is clear and translucent, and the membrane concentration of the subsequent membrane is formed to effectively protect;

2. The nanofiltration process not only concentrates the product, but also separates impurities such as ions and ash;

3. The membrane energy consumption is low, about 1/5 of the evaporation method;

4. The whole process is simple in process, convenient in operation and can realize automatic control.

The integrated membrane technology uses a programmable process control system, complemented by a partial on-site indicator to automate the process control process. The programmable PLC system realizes automatic operation and monitoring operation, controls the production process, and introduces the process parameters of each section into the control system of the control room for centralized monitoring, thereby realizing digital intelligent production.

Jiuwu Hi-tech has been developing and applying the membrane integration process of enzymatic peptides for more than 10 years, and has a deep understanding and experience. According to different molecular weight products of customers, different molecular weight cutting membranes are used to separate and purify peptides with different molecular weights, improve the quality and yield of peptide products, simplify the process and save energy. With the increasing influence of peptides on the international market and the diversification of development models, Jiuwu Hi-tech will also contribute to the development of the peptide industry chain through in-depth research and development in membrane materials, membrane equipment and membrane technology.

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