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Amino Acid Fermentaion Broth Clarification

Amino acids are the basic unit of protein composition, most of the amino acid processes are through fermentation method. Fermentation broth is an extremely complex system and contains microbial cells, metabolites, unconsumed medium, etc.

The traditional method of amino acid production processes such as centrifuge and plate and frame pressure filtration, the filtrate quality is poor. Soluble protein, sugar, peptide, pigment and other impurities can not be effectively removed, increasing the processing load of the subsequent process, affecting overall yield of the extraction process, also affecting quality of final product.

Amino acids: lysine, glutamic acid, phenylalanine, threonine, tryptophan, glycine, arginine, glutamine, theanine, proline, valine, isoleucine

Ceramic membrane integrated organic membrane filtration process system has the unique advantages of high separation precision, maintaining high-throughput filtration and high product yield, less waste water, less cleaning frequency, without adding additives and achieved desalination and preconcentration of the target product which has been successfully applied to bio-fermentation enterprises such as glutamic acid production (sodium), lysine production, threonine production, tryptophan production, valine production and so on compared with traditional process.

Process features
Compared with traditional separation process, the quality of the filtrate is guaranteed due to high separation accuracy and uniform pore size distribution; high-throughput filtration can be maintained, and cleaning frequency is reduced because membranes have smooth surface to result in membrane flux slowly decay; Improve product yield, reduce the amount of wastewater, pore size diversification, suitable for different types of materials processing requirements;

Without adding additives, concentrated material (mycelium, protein, etc.) can be used as feed recovery;

95% of fermentation of bacterial cells integrity and activity can be guaranteed;

Organic membrane filtration is used to to remove small molecules protein or peptides, nucleic acids, dyes and other impurities from permeation generated by ceramic membrane and improve product purity and reduce sewage treatment load;

Achieve the desalination and preconcentration of the target product, and reduce the production load of subsequent processing technologies such as solvent extraction, ion exchange and evaporation concentration.

Xanthan Gum

Xanthan gum is a polysaccharide with a wide variety of uses, including as a common food additive. It is a powerful thickening agent, and also has uses as a stabilizer to prevent ingredients from separating.

Xanthan gum is known as "industrial MSG", is the largest and widely used microbial polysaccharide in the world at present.

Use of membrane filtration and separation operations allows concentration and purification of substances such as xanthan with recycling of permeate. It also provides an inexpensive and reliable way to remove water and low molecular weight impurities. Fractionation, concentration, and purification of gelatins via membrane operations result in gentler processing to avoid denaturing the gelatine.


Vitamin C production has two fermentation processes: Reichstein method and two-step fermentation. Impurities in fermentation broth are removed by the use of heat precipitation method for the above two traditional vitamin C extraction process. After the fermentation broth was allowed to settle, it was separated by a cation exchange resin. However, the fermentation broth is passed directly through the ion exchange resin, which can cause serious contamination of the resin surface and reduce the exchange capacity.

Modern extraction process generally applies ultrafiltration membrane gulonic acid fermentation broth production, because the broth protein molecular weight range is generally 1-10kDa, so to choose a certain molecular weight cut-off can remove most of the protein and some macromolecular impurities in place of the traditional heating method to remove protein.

Compared with the traditional process, Gulonic acid fermentation broth has obvious advantages: simple process, fully automatic operation and good stability; the yield is improved, generally reach more than 99% during membrane filtration for removing impurities. At present, integrated membrane technology including ceramic membrane, ultrafiltration & amp; nanofiltration membrane is applied to vitamin C production enterprises because membrane process has more important feature is the separation of relatively high accuracy, which can be verified from the fermentation broth protein removal rate.

Vitamins: Vitamin C, vitamin B2, vitamin B12 and so on

Related Application

JIUWU Membrane Products

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